Hepatitis A virus (ATCC® VR-2093)

Classification: Picornaviridae, Hepatovirus  /  Product Format: frozen

Classification Picornaviridae, Hepatovirus
Agent Hepatitis A virus
Strain HM 175 (uncloned)
Applications
testing virucides
Biosafety Level 2
Isolation
Human stool specimens from outbreak of virus, Australia
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Effect on Host
Yes, in vitro effects: Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate
Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate.
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:  FRhK-4 (ATCC CRL-1688)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 21 to 28 days; For best results cells should be 24 to 48 hours old and 60-80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate
Subtle CPE may be missed. Monolayer does not fill in, cells round and deteriorate.
Name of Depositor NIH
Source
Human stool specimens from outbreak of virus, Australia
References

Feinstone SM, et al. Isolation of hepatitis A virus strain HM-175. US Patent 4,532,215 dated Jul 30 1985

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Daemer RJ, et al. Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage. Infect. Immun. 32: 383-393, 1981. PubMed: 6260685

or colder

or colder