TF-1 (ATCC® CRL-2003)

Organism: Homo sapiens, human  /  Cell Type: erythroblast  /  Tissue: bone marrow  /  Disease: erythroleukemia

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Organism Homo sapiens, human
Tissue bone marrow
Cell Type erythroblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease erythroleukemia
Age 35 years
Gender male
Ethnicity Japanese
Applications
This cell line provides a good system for investigating the proliferation and differentiation of myeloid progenitor cells.
Storage Conditions liquid nitrogen vapor phase
Derivation
The TF-1 cell line was established by T. Kitamura, et al. in October 1987 from a heparinized bone marrow aspiration sample from a 35 year old Japanese male with severe pancytopenia. ­
Clinical Data
male
Japanese
35 years

Comments

The cells are completely dependent on interleukin 3 (IL-3) orgranulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. 

The cells DO NOT RESPOND to interleukin 5 (IL-5). TF-1 cells respond to a variety of other lymphokines and cytokines such as interleukin 1 (IL-1), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 9 (IL-9),Interleukin 11 (IL-11), interleukin 13 (IL-13), stem cell factor (SCF), leukemia inhibitory factor (LIF) and nerve growth factor (NGF). TF-1 cells do not express glycophorin A or carbonyl anhydrase I. 

The morphological and cytochemical features, and the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. Hemin and delta-aminolevulinic acid induce hemoglobin synthesis, and TPA induces dramatic differentiation of the TF-1 cells into macrophage-like cells. 

The TF-1 cell line is unique because of its responsiveness to multiple cytokines.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2 ng/ml recombinant human GM-CSF; fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 104 viable cells/mL. Do not allow the cell concentration to reach 7 X 10cells/mL.
Interval: Maintain cultures between 3 X 104 and 5 X 105 viable cells/mL
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 13,14
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
Population Doubling Time 22 hours
Name of Depositor T Kitamura
Year of Origin 1987
References

Kitamura T, et al. IL-1 up-regulates the expression of cytokine receptors on a factor- dependent human hemopoietic cell line, TF-1. Int. Immunol. 3: 571-577, 1991. PubMed: 1832294

Kitamura T, et al. Identification and analysis of human erythropoietin receptors on a factor-dependent cell line, TF-1. Blood 73: 375-380, 1989. PubMed: 2537111

Kitamura T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334, 1989. PubMed: 2663885

Borellini F, Glazer RI. Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1. J. Biol. Chem. 268: 7923-7928, 1993. PubMed: 8463313

Schulte T, et al. Molecular characterization and functional analysis of murine interleukin 4 receptor allotypes. J. Exp. Med. 186: 1419-1429, 1997. PubMed: 9348299

Mueller CG, et al. Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells. J. Exp. Med. 186: 655-663, 1997. PubMed: 9271581

Limon A, et al. High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells. Blood 90: 3316-3321, 1997. PubMed: 9345013

Hu X, et al. Prolonged activation of the mitogen-activated protein kinase pathway is required for macrophage-like differentiation of a human myeloid leukemic cell line. Cell Growth Differ. 11: 191-200, 2000. PubMed: 10775036

USP Monographs: Sargramostim. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kitamura T, et al. IL-1 up-regulates the expression of cytokine receptors on a factor- dependent human hemopoietic cell line, TF-1. Int. Immunol. 3: 571-577, 1991. PubMed: 1832294

Kitamura T, et al. Identification and analysis of human erythropoietin receptors on a factor-dependent cell line, TF-1. Blood 73: 375-380, 1989. PubMed: 2537111

Kitamura T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334, 1989. PubMed: 2663885

Borellini F, Glazer RI. Induction of Sp1-p53 DNA-binding heterocomplexes during granulocyte/macrophage colony-stimulating factor-dependent proliferation in human erythroleukemia cell line TF-1. J. Biol. Chem. 268: 7923-7928, 1993. PubMed: 8463313

Schulte T, et al. Molecular characterization and functional analysis of murine interleukin 4 receptor allotypes. J. Exp. Med. 186: 1419-1429, 1997. PubMed: 9348299

Mueller CG, et al. Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells. J. Exp. Med. 186: 655-663, 1997. PubMed: 9271581

Limon A, et al. High-titer retroviral vectors containing the enhanced green fluorescent protein gene for efficient expression in hematopoietic cells. Blood 90: 3316-3321, 1997. PubMed: 9345013

Hu X, et al. Prolonged activation of the mitogen-activated protein kinase pathway is required for macrophage-like differentiation of a human myeloid leukemic cell line. Cell Growth Differ. 11: 191-200, 2000. PubMed: 10775036

USP Monographs: Sargramostim. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011