Bacillus atrophaeus Nakamura (ATCC® 51189)

Strain Designations: NCTC 10073 [Camp Detrick, DSM 2277, NCIB 8649, NRCB-467]  /  Type Strain: no  /  Biosafety Level: 1

Permits And Restrictions

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Deposited As Bacillus subtilis (Ehrenberg) Cohn
Strain Designations NCTC 10073 [Camp Detrick, DSM 2277, NCIB 8649, NRCB-467]
Media testing
Sterility testing
Ethylene oxide sterilization control
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Preceptrol® no
Type Strain no
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 30.0°C
Name of Depositor NCTC
Chain of Custody
ATCC <<--NCTC<<--J.C. Kelsey (Bacillus globigii) <<--- C.R. Phillips

Beeby MM, Whitehouse CE. A bacterial spore test piece for the control of ethylene oxide sterilization. J. Appl. Bacteriol. 28: 349-360, 1965. PubMed: 4954908

Kelsey JC. Use of gaseous antimicrobial agents with special reference to ethylene oxide. J. Appl. Bacteriol. 30: 92-100, 1967. PubMed: 6045112

Fritze D, Pukall R. Reclassification of bioindicator strains Bacillus subtilis DSM 675 and Bacillus subtilis DSM 2277 as Bacillus atrophaeus. Int. J. Syst. Evol. Microbiol. 51: 35-37, 2001. PubMed: 11211269

Plastics collapsible containers for human blood and blood components--Part 1: Conventional containers. Annex C. Biological tests.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 3826-1:2003.

Sterilization of health care products--Biological indicators--Part 2: Biological indicators for ethylene oxide sterilization. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11138-2:1994.

Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14698-1:2003.

Plastic containers for intravenous injection. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 15747:2003.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation