Human herpesvirus 1 (ATCC® VR-733)

Classification: Herpesviridae, Simplexvirus  /  Product Format: 1 mL per vial

Classification Herpesviridae, Simplexvirus
Agent Human herpesvirus 1
Strain F
Common Name Herpes simplex virus 1
Applications
Susceptibility testing
Testing virucides
Biosafety Level 2
Product Format 1 mL per vial
Storage Conditions -70°C or colder
Images VR-733 Images
Comments Deposited as Herpes simplex virus 1.
This strain exhibits slight cross reactivity with certain Human herpesvirus 2 monoclonals.
Effect on Host
CPE, refractile rounding and cell clumping
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 34°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host
CPE, refractile rounding and cell clumping
Name of Depositor B Roizman, Institut Merieux
Special Collection NCRR Contract
Source Facial vesicle
References

Antiviral Susceptibility Testing: Herpes Simplex Virus by Plaque Reduction Assay; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M33-A.

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Ejercito PM, et al. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J. Gen. Virol. 2: 357-364, 1968. PubMed: 4300104

Frankel N, Roizman B. Herpes simplex virus: genome size and redundancy studied by renaturation kinetics. J. Virol. 8: 591-593, 1971. PubMed: 4331657

Kieff ED, et al. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J. Virol. 8: 125-132, 1971. PubMed: 4329966

Kieff ED, et al. Genetic relatedness of type 1 and type 2 herpes simplex viruses. J. Virol. 9: 738-745, 1972. PubMed: 4337161

Roizman B. Perspectives in Virology VIII. New York, NY: Academic Press; .