Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 43300)

Strain Designations: F-182  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations F-182
Application
Evaluation of Mueller-Hinton agar
Resistant to methicillin
Resistant to oxacillin
Susceptibility disc testing
Susceptibility testing
Drug discovery
Enteric Research
Isolation
Clinical isolate, Kansas
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Antibiotic Resistance
Resistant to methicillin
Resistant to oxacillin
Comments
Absence of pvl gene confirmed by PCR.
Medium Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor C Thornsberry
Isolation
Clinical isolate, Kansas
References

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

Baker CN, Tenover FC. Evaluation of Alamar colorimetric broth microdilution susceptibility testing method for staphylocci and enterococci. J. Clin. Microbiol. 34: 2654-2659, 1996. PubMed: 8897159

Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P.

Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002

MicroScan Dried Gram Positive Panel QC Set. Dade/Siemens.

BD GeneOhm MRSA ACP. BD Becton Dickinson.

BD GeneOhm StaphSR. BD Becton Dickinson.

BD MAX™ MRSA Assay. BD Becton Dickinson.

BD MAX™ StaphSR Assay. BD Becton Dickinson.

Cross References

Nucleotide (GenBank) : AF298796 Staphylococcus aureus translation elongation factor Tu (tuf) gene, partial cds.