Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 43300)

Strain Designations: F-182  /  Type Strain: no  /  Biosafety Level: 2

Permits And Restrictions

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Strain Designations F-182
Application
Evaluation of Mueller-Hinton agar
Resistant to methicillin
Resistant to oxacillin
Susceptibility disc testing
Susceptibility testing
Drug discovery
Enteric Research
Isolation
Clinical isolate, Kansas
Type Strain no
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Antibiotic Resistance
Resistant to methicillin
Resistant to oxacillin
Comments
Absence of pvl gene confirmed by PCR.
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor C Thornsberry
Isolation
Clinical isolate, Kansas
References

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

Baker CN, Tenover FC. Evaluation of Alamar colorimetric broth microdilution susceptibility testing method for staphylocci and enterococci. J. Clin. Microbiol. 34: 2654-2659, 1996. PubMed: 8897159

Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P.

Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002

MicroScan Dried Gram Positive Panel QC Set. Dade/Siemens.

BD GeneOhm MRSA ACP. BD Becton Dickinson.

BD GeneOhm StaphSR. BD Becton Dickinson.

BD MAX™ MRSA Assay. BD Becton Dickinson.

BD MAX™ StaphSR Assay. BD Becton Dickinson.

Cross References

Nucleotide (GenBank) : AF298796 Staphylococcus aureus translation elongation factor Tu (tuf) gene, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation