Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers (ATCC® 25285)

Strain Designations: VPI 2553 [EN-2; NCTC 9343]  /  Type Strain: yes  /  Biosafety Level: 2

Strain Designations VPI 2553 [EN-2; NCTC 9343]
Application
Media testing
Quality control strain
Susceptibility testing
Quality control strain for BBL and MicroScan® products
Pharmaceutical and Personal Care
Isolation
Appendix abscess
Type Strain yes
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Genome Sequenced Strain

Bacteroides fragilis NCTC 9343, complete genome

Type Strain yes
Comments
Genome sequenced strain
Medium ATCC® Medium 1490: Modified chopped meat medium
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor WE Moore
Chain of Custody
ATCC <-- WE Moore <-- A Sonnenwirth EN-2 <-- NCTC 9343 <-- L Garrod
Isolation
Appendix abscess
References

Cato EP, Johnson JL. Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetaiotaomicron, and B. vulgatus: Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers. Int. J. Syst. Bacteriol. 26: 230-237, 1976.

Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7.

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.

Takahata M, Nishino T. Antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects. Chemotherapy 43: 153-158, 1997. PubMed: 9142454

BD Crystal ANR QC Set. BD Becton Dickinson; Crystal

MicroScan Rapid Anaerobe ID QC Set. Dade/Siemens.

Sensititre™ Anaerobe MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.

Medical microbiology--Culture media -- Part 4: Transport systems for specimens containing bacteria. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-4: 2003, 2003

Cross References

Nucleotide (GenBank) : X83935 B.fragilis 16S rRNA (ATCC 25285T).

Nucleotide (GenBank) : AX110196 Sequence 929 from Patent WO0123604.

Nucleotide (GenBank) : AF280705 Bacteroides fragilis isolate ATCC 25285 RecA (recA) gene, partial cds.

Nucleotide (GenBank) : U05887 Bacteroides fragilis ATCC 25285 beta-lactamase (cepA-85) gene, complete cds.

Nucleotide (GenBank) : AF280642 Bacteroides fragilis isolate ATCC 25285 glutamine synthetase (glnA) gene, partial cds.

Nucleotide (GenBank) : CR626927 Bacteroides fragilis NCTC 9343, complete genome