Clostridium perfringens (Veillon and Zuber) Hauduroy et al. (ATCC® 12916)

Strain Designations: NCTC 8238 [281/50]  /  Type Strain: no  /  Biosafety Level: 2

Deposited As Clostridium welchii
Strain Designations NCTC 8238 [281/50]
Application
Media testing
Enteric Research
Isolation
Boiled salt beef
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Comments
Agglutinating type 2
Type A
The presence of cpa and cpe genes was confirmed by PCR.
This strain does not produce epsilon toxin
Toxins

This strain does not produce epsilon toxin.

Medium Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor NCTC
Chain of Custody
ATCC <-- NCTC <-- B. Hobbs 281/50
Isolation
Boiled salt beef
References

Hobbs BC, et al. Clostridium welchii food poisoning. J. Hyg. (Lond). 51(1): 75-101, 1953. PubMed: 13044934

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.