Gallid herpesvirus 3 (ATCC® VR-2001)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen

Classification Herpesviridae, Mardivirus
Agent Gallid herpesvirus 3
Strain SB-1 (clone of SB)
Applications
NOTE: This material is cited in a U.S. and/or other Patent Application and may not be used to infringe the patent claims.
Will protect against MDV and non-virus producing JM tumor transplant.
Biosafety Level 2
Isolation
chicken (S-strain) peripheral blood lymphocytes
Product Format frozen
Storage Conditions  Vapor phase of liquid nitrogen
Comments
Non-oncogenic, cell-associated. Will protect against MDV and non-virus producing JM tumor transplant. NOTE: This material is cited in a U.S. and/or other Patent Application and may not be used to infringe the patent claims.
Note that SB-1 is strictly cell-associated, damage to the infected cells will cause loss of titer. Thaw frozen ampule in 27C waterbath; dilute content slowly in 9.0 ml medium or PBS; pellet cells (10min at 400Xg); resuspend in culture medium.
Effect on Host
 CPE, cell rounding and syncytia formation
Recommended Host
chicken embryo fibroblasts
Growth Conditions
Temperature: 38°C to 39°C
Incubation:  Incubate infected culture for 7 days at 38-39°C in a humidified 5% CO2 atmosphere, until CPE are well advance throughout.

Cryopreservation
Storage temperature: Liquid nitrogen
Effect on Host
 CPE, cell rounding and syncytia formation
Name of Depositor BW Calnek
Source
chicken (S-strain) peripheral blood lymphocytes
References

Schat KA, Calnek BW. Characterization of an apparently nononcogenic Marek's disease. J. Natl. Cancer Inst. 60: 1075-1082, 1978. PubMed: 76680