Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 17858-FZ)

Strain Designations: 1253  /  Type Strain: no  /  Biosafety Level: 2

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Strain Designations 1253
Type Strain no
Biosafety Level 2
Product Format frozen
Shipping Information Frozen
Preceptrol® no
Genotype Toxinotype: 0
Type Strain no
Comments
Preparation of Clostridium difficile strain 1253 ATCC® 17858-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Toxinotype 0
Ribotype 054
Presence of tcdA and tcdB genes confirmed by PCR.
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Binary toxin gene cdtB not amplified by PCR
Medium ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture 80% N2 -10% CO2 -10% H2
Genotype Toxinotype: 0
Name of Depositor LS McClung
References

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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