• remove Product Category: Primary Cells

Specialty Cell Culture Media

  • TransfeX™ Transfection Reagent (ATCC® ACS-4005)

    ATCC® Number: ACS-4005
    Quantity: 500 μL
    Storage: Store at 2°C to 8°C
    Applications:

    Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

    For-Profit:   $225.00 Non-Profit:   $225.00

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    Product Format: 500 μL
    Comments:

    Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality.

  • Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs (ATCC® PCS-500-053)

    ATCC® Number: PCS-500-053
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications: The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs contains medium and reagents designed to induce adipogenesis in actively proliferating Bone Marrow and Umbilical-Derived Mesenchymal Stem Cells with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components: 

    1. One vial containing 1 mL of of ADM Supplement BMMSC/UCMSC for use in initiation of adipocyte differentiation 
    2. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation 

    * The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format.

    For-Profit:   $159.00 Non-Profit:   $159.00

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  • For-Profit:   $76.00 Non-Profit:   $76.00

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  • Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030)

    ATCC® Number: PCS-600-030
    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

    ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $80.00 Non-Profit:   $80.00

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  • HEKPlus Boost Solution (ATCC® ACS-4003)

    ATCC® Number: ACS-4003
    Quantity: 50 mL
    Storage: Store at 2°C to 8°C. Do not freeze. Protect from light.

    For-Profit:   $35.00 Non-Profit:   $35.00

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    Product Format: 50 mL
    Comments: The addition of peptone following transfection has been shown to increase extracellular protein expression yields in transient transfection protocols.

    HEKPlus Boost Solution should be added post transfection to a final concentration of 10% in complete HEKPlus SFM. The solution contains 0.1% Poloxamer 188, a surfactant shown to enhance cell yield and reduce cell aggregation. HEKPlus Boost Solution also contains human transferrin and recombinant human insulin.

    This solution has been optimized for use with ATCC® HEK293T/17 SF suspension cells (ATCC ACS-4500), HEKPlus SFM medium (ATCC ACS-4002) and GeneXPlus transfection reagent (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • GeneXPlus Transfection Reagent (ATCC® ACS-4004)

    ATCC® Number: ACS-4004
    Quantity: 1 mL
    Storage: -20°C
    Applications:

    Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266).

    For-Profit:   $350.00 Non-Profit:   $350.00

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    Product Format: 1 mL
    Comments:

    This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

     

    GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • Dermal Cell Basal Medium (ATCC® PCS-200-030)

    ATCC® Number: PCS-200-030
    Quantity: 485 ml
    Storage: 2 to 8°C, protect from light
    Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions™ system.

    This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. 

    To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCC PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCC PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

    To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCC PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCC PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCC PCS-200-041) or the Adult Melanocyte Growth Kit (ATCC PCS-200-042).

    Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

     

    For-Profit:   $50.00 Non-Profit:   $50.00

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  • For-Profit:   $26.00 Non-Profit:   $26.00

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  • VeroPlus SFM (ATCC® ACS-4001)

    ATCC® Number: ACS-4001
    Quantity: 500 mL
    Storage: Store product at 2°C to 8°C. Do not freeze.

    For-Profit:   $40.00 Non-Profit:   $40.00

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    Product Format: 500 mL
    Comments:

    Each lot of VeroPlus SFM is tested functionally for growth characteristics and morphology with Vero cells. Additionally, the media is assayed for pH, osmolality, endotoxins and sterility.

    Supplementing with L- Glutamine or L-alanyl-L-Glutamine is required before use.

  • For-Profit:   $26.00 Non-Profit:   $26.00

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