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Specialty Cell Culture Media

  • TransfeX™ Transfection Reagent (ATCC® ACS-4005)

    ATCC® Number: ACS-4005
    Quantity: 500 μL
    Storage: Store at 2°C to 8°C
    Applications:

    Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.

    For-Profit:   $225.00 Non-Profit:   $225.00

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    Product Format: 500 μL
    Comments:

    Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality.

  • Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs (ATCC® PCS-500-053)

    ATCC® Number: PCS-500-053
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)
    Applications: The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs contains medium and reagents designed to induce adipogenesis in actively proliferating Bone Marrow and Umbilical-Derived Mesenchymal Stem Cells with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components: 

    1. One vial containing 1 mL of of ADM Supplement BMMSC/UCMSC for use in initiation of adipocyte differentiation 
    2. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation 

    * The Adipocyte Differentiation Toolkit for Bone Marrow and Umbilical-Derived MSCs provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format.

    For-Profit:   $159.00 Non-Profit:   $159.00

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  • Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs (ATCC® PCS-500-041)

    ATCC® Number: PCS-500-041
    Quantity: 1 kit
    Storage: -20°C (or -70°C for long-term storage)

    For-Profit:   $118.00 Non-Profit:   $118.00

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    Product Format: frozen 1 kit
    Comments:

    The Mesenchymal Stem Cell Growth Kit for Bone Marrow-derived MSCs contains components that when added to Mesenchymal Stem Cell Basal Medium for Adipose, Umbilical and Bone Marrow-derived MSCs (ATCC PCS-500-030) create a complete cell culture environment for mesenchymal stem cells isolated from normal human bone marrow. The final concentration for each component in complete mesenchymal stem cell growth medium is as follows:

    • rh FGF basic: 125 pg/mL
    • rh IGF-1: 15 ng/mL 
    • Fetal Bovine Serum: 7% 
    • L-Alanyl-L-Glutamine: 2.4 mM   
  • Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030)

    ATCC® Number: PCS-600-030
    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

    ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $80.00 Non-Profit:   $80.00

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  • GeneXPlus Transfection Reagent (ATCC® ACS-4004)

    ATCC® Number: ACS-4004
    Quantity: 1 mL
    Storage: -20°C
    Applications:

    Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266).

    For-Profit:   $350.00 Non-Profit:   $350.00

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    Product Format: 1 mL
    Comments:

    This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.

     

    GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

  • VeroPlus SFM (ATCC® ACS-4001)

    ATCC® Number: ACS-4001
    Quantity: 500 mL
    Storage: Store product at 2°C to 8°C. Do not freeze.

    For-Profit:   $40.00 Non-Profit:   $40.00

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    Product Format: 500 mL
    Comments:

    Each lot of VeroPlus SFM is tested functionally for growth characteristics and morphology with Vero cells. Additionally, the media is assayed for pH, osmolality, endotoxins and sterility.

    Supplementing with L- Glutamine or L-alanyl-L-Glutamine is required before use.

  • Stem Cell Dissociation Reagent (ATCC® ACS-3010)

    ATCC® Number: ACS-3010
    Quantity: 250 mg
    Storage: 2°C to 8°C

    For-Profit:   $90.00 Non-Profit:   $90.00

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    Product Format: 250 mg
    Comments:

    Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020
    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $50.00 Non-Profit:   $50.00

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    Product Format: frozen 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Pluripotent Stem Cell SFM XF/FF (ATCC® ACS-3002)

    ATCC® Number: ACS-3002
    Storage: Basal Medium is stored at -20°C. Growth Supplement is stored at 2°C to 8°C.

    For-Profit:   $230.00 Non-Profit:   $230.00

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    Product Format: frozen
    Comments:
    Media components are tested for pH, osmolality, endotoxins and sterility. Each lot of Pluripotent Stem Cell SFM XF/FF is tested functionally with hESCs and hiPSCs to ensure that pluripotency is maintained as determined by expression of pluripotent markers.