P3/NSI/1-Ag4-1 [NS-1] (ATCC® TIB-18)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: plasmacytoma; myeloma

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Strain BALB/c
Applications
Kappa chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.
Storage Conditions liqid nitrogen vapor phase
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Derivation
This is a non-secreting clone of P3X63Ag8 (ATCC TIB-9).
Antigen Expression
Antigen expression: H-2d
Genes Expressed
immunoglobulin (not secreted),H-2d
Cellular Products
immunoglobulin (not secreted)
Comments
Kappa chains are synthesized but not secreted.
The cells are resistant to 0.1 mM 8-azaguanine and do not grow in HAT medium.
The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL. Do not allow the cell density to exceed 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype kappa light chain
Name of Depositor G Kohler, C Milstein
References

Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938

Kohler G, et al. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur. J. Immunol. 6: 292-295, 1976. PubMed: 825374

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377

Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851

Cowan NJ, et al. Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. J. Mol. Biol. 90: 691-701, 1974. PubMed: 4449137

Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742

Cross References

Nucleotide (GenBank) : AF248032 Mus musculus coatomer protein alpha subunit precursor, mRNA, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938

Kohler G, et al. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur. J. Immunol. 6: 292-295, 1976. PubMed: 825374

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Kohler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377

Sato JD, et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed: 3209588

Ramasamy R, et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed: 4545851

Cowan NJ, et al. Intracellular immunoglobulin chain synthesis in non-secreting variants of a mouse myeloma: detection of inactive light-chain messenger RNA. J. Mol. Biol. 90: 691-701, 1974. PubMed: 4449137

Condie R, et al. Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2. J. Biol. Chem. 271: 13175-13183, 1996. PubMed: 8662742