HuNS1 (ATCC® CRL-8644)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Disease: myeloma

Organism Homo sapiens, human
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease myeloma
Gender male
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).
Clinical Data male
Comments

The cells are HAT sensitive and are negative for Epstein-Barr virus.

According to information provided by the patent depositor (US Patent 4,720,459 dated Jan 19 1988), this cell line did not secrete an immunoglobulin. However, recent testing at ATCC has confirmed the presence of IgG.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete Culture Medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 10,12
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,19
Name of Depositor Medical College of Wisconsin Research Foundation, Inc.
References

Winkelhake JL. Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

Winkelhake JL. Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.