Mammalian orthoreovirus 1 (ATCC® VR-230)

Classification: Reoviridae, Orthoreovirus  /  Product Format: frozen

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Classification Reoviridae, Orthoreovirus
Agent Mammalian orthoreovirus 1
Strain Lang
Biosafety Level 2
Isolation
Rectal swab from healthy child, Ohio, 1953
Product Format frozen
Storage Conditions -70°C or colder
Comments
For passage cell cultures should be harvested after complete involvement of cell sheet. Culture fluid contains a group-specific CF antigen.
REFERENCE STRAIN: Sabin, A.B., Science (Washington, DC) 130: 1387, 1959.
Effect on Host
Yes, in vitro effects: Cytopathic effects (granulated degeneration of cells and then destruction of entire cell sheet)
CPE; granulated degeneration of cells and then destruction of entire cell sheet
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host: LLC-MK2 (ATCC CCL-7.1)

Growth Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Duration: 2-6 days
Effect on Host
Yes, in vitro effects: Cytopathic effects (granulated degeneration of cells and then destruction of entire cell sheet)
CPE; granulated degeneration of cells and then destruction of entire cell sheet
Name of Depositor EH Lennette
Source
Rectal swab from healthy child, Ohio, 1953
Year of Origin 1953
References

Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336

Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578

Cross References

Nucleotide (GenBank) : AJ133089 Reovirus type 1 S2 gene, partial, strain Lang.

Nucleotide (GenBank) : M13139 Reovirus (Lang strain) s4 mRNA encoding the major capsid surface protein sigma-3, complete cds.

Nucleotide (GenBank) : M14325 Reovirus (Lang strain) s3 gene encoding the nonstructural RNA-binding protein sigma-NS, complete cds.

Nucleotide (GenBank) : M14779 Reovirus (Lang strain) s1 mRNA encoding the minor capsid cell attachment protein sigma-1a and the nonstructural protein sigma-ibNS, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation