Simian adenovirus 7 (ATCC® VR-201)

Classification: Adenoviridae, Mastadenovirus  /  Product Format: frozen

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Classification Adenoviridae, Mastadenovirus
Agent Simian adenovirus 7
Strain 8045-2WN
Applications
Respiratory research
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder
Comments Simian Virus Group I (Hull).
REFERENCE STRAIN: Pereira, H.G. et al., Virology 20:613, 1963.
Originally deposited as SV25
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
LLC-MK2 (ATCC CCL-7.1)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-14 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor RN Hull
Source Tissue cultures prepared from pooled rhesus monkey kidney cells
References

Minner JR, et al. New viral agents recovered from tissue cultures of monkey kidney cells. III. Recovery of additional agents both from cultures of monkey tissues and directly from tissues and excreta. Am. J. Hyg. 68: 31-44, 1958. PubMed: 13559209

Roy S, et al. Construction of gene transfer vectors based on simian adenovirus 7. J. Gen. Virol. 92(8): 1749-1753, 2011. PubMed: 21562118

Cross References

Nucleotide (GenBank) : DQ792570.1 Simian adenovirus 7, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation