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Cell Biology

  • Dulbecco's Phosphate Buffered Saline (D-PBS), 1X (ATCC® 30-2200)

    ATCC® Number: 30-2200
    Quantity: 500 mL
    Storage: Store Dulbecco's PBS at ambient temperatures, between 15°C and 30°C.
    Applications:

    To be used for washing cells,  as a diluent, or as an inorganic base for standard media preparation.

    For-Profit:   $15.00 Non-Profit:   $15.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    Formulation contains no calcium or magnesium.

  • DMEM: F-12 Medium (ATCC® 30-2006)

    ATCC® Number: 30-2006
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Hank's Balanced Salt Solution (HBSS), 1X (ATCC® 30-2213)

    ATCC® Number: 30-2213
    Quantity: 500 mL
    Storage: Store Hank's BSS at ambient temperatures, between 15°C and 30°C
    Applications: For washing cells, for use as a diluent, or for use as an inorganic base for standard media preparation.

    For-Profit:   $14.00 Non-Profit:   $14.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    Contains no calcium, magnesium, or phenol red.
  • Water, Cell Culture Grade (ATCC® 30-2205)

    ATCC® Number: 30-2205
    Quantity: 500 mL
    Storage: Store cell culture grade water at room temperature (15°C to 30°C). Shelf life of 36 months when properly stored.
    Applications:

    For use as a diluent or for reconstituting powdered media and salt solutions.

    For-Profit:   $11.00 Non-Profit:   $11.00

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    Product Format: 500 mL
    Comments:
    Cell culture tested.
  • Trypsin-EDTA Solution, 1X (ATCC® 30-2101)

    ATCC® Number: 30-2101
    Quantity: 100 mL
    Storage: Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications:

    0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.

    For-Profit:   $13.00 Non-Profit:   $13.00

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    Product Format: frozen 100 mL
    Comments: NOTE:  Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner.  For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service. ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.
  • CoolCell® LX Alcohol-Free Cryopreservation Container (ATCC® ACS-6000)

    ATCC® Number: ACS-6000
    Applications:

    The CoolCell® LX container, in combination with a -80°C freezer or dry ice locker, will provide the freezing rate of -1°C per minute that is ideal for cryo-preservation of most cultured cell lines.  

    For-Profit:   $149.00 Non-Profit:   $149.00

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  • Fetal Bovine Serum (ATCC® 30-2020)

    ATCC® Number: 30-2020
    Quantity: 500 mL
    Storage: Store fetal bovine serum at -20°C or colder. Do not store frozen fetal bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing fetal bovine serum in aliquots. Fetal bovine serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

    For-Profit:   $324.00 Non-Profit:   $324.00

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  • Hybri-Care Medium (ATCC® 46-X)

    ATCC® Number: 46-X
    Quantity: 1 Package (prepares 1L of medium)
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines.
    A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 1 Package (prepares 1L of medium)
    Comments:
    Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning.

    When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection.

    Ozato, K., et al. J. Immunology 124: 533-540, 1980.
  • Calf Bovine Serum (ATCC® 30-2031)

    ATCC® Number: 30-2031
    Quantity: 100 mL
    Storage: Store calf bovine serum at -20°C or colder. Do not store frozen calf bovine serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing calf bovine serum in aliquots. Calf bovine serum is supplied in PETG plastic bottles.
    Applications: Iron supplemented and triple filtered through 0.1 μm filters. Each lot of calf bovine serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Calf bovine serum is manufactured from bovine blood collected in USDA-inspected abattoirs located in the United States. Serum has not come from cattle born, raised, shipped through, or slaughtered in countries where BSE is known to exist.

    For-Profit:   $21.00 Non-Profit:   $21.00

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  • Horse Serum (ATCC® 30-2041)

    ATCC® Number: 30-2041
    Quantity: 100 mL
    Storage: Store horse serum at -20°C or colder. Do not store frozen horse serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing horse serum in aliquots. Horse serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of horse serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Horse serum is manufactured from blood collected from select donor horse herds maintained within the United States.

    For-Profit:   $19.00 Non-Profit:   $19.00

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