DNA from HCMV strain Towne [ATCC VR-977] (ATCC® VR-977D)

Organism: Human herpesvirus 5, Human cytomegalovirus  / 

Product Format frozen

PRODUCT INFORMATION:

Total DNA from infected cells, >1 µg/mL in 1X TE buffer

100 µL per vial, shipped frozen

OD260/OD280: > 1.7

Storage Conditions -70°C or colder
Applications
It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Comments
This product is a preparation of viral nucleic acids, including virus genomic DNA, in total DNA isolated from MRC-5 cells (ATCC CCL-171) infected with Human herpesvirus 5 (HCMV) strain Towne (ATCC VR-977). It is supplied at 100 µl per vial, shipped frozen. It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Biosafety Level 1
Quality Control Specifications
1. Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).

2. Concentration is verified as > 1 µg/mL DNA by Picogreen® assay.

3. Purity is confirmed by OD260/OD280 ratio (> 1.7) of bulk DNA prior to dilution for product filling.

4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis 20 ul of product on a 0.8% agarose gel, visualized by ethidium bromide staining.

5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.

6. Identity is confirmed by sequencing of ~300 base pairs of the PCR amplicon.

7. Absence of infectious virus is verified by incubation of 10% of each lot with susceptible host cells under appropriate conditions for a period of 7-14 days.