Chlamydophila psittaci (Lillie) Everett et al. (ATCC® VR-351)

Classification: Chlamydiaceae  /  Product Format: frozen

Classification Chlamydiaceae
Deposited As Ornithosis
Agent Chlamydophila psittaci (Lillie) Everett et al.
Strain Ornithosis strain Texas Turkey [strain Texas turkey]
Applications
Addition of cycloheximide recommended for passage in cell culture.
Biosafety Level 3
Isolation
turkey spleen, Texas
Product Format frozen
Storage Conditions -70°C or colder
Comments
Centrifugation (2000 X g for 1 hr.) and addition of cycloheximide recommended for passage in cell culture. *Requests for this agent must carry signed statement assuming all risks and responsibility for lab handling.
Triturate fluid several times to disperse chlamydia. For cell culture, centrifuge inoculumn, adsorb for 1 hour and feed with HEPES buffered culture medium.
Effect on Host
Cytopathic effects (inclusions) in tissue culture
Death of embryo
Recommended Host
McCoy cells (ATCC CRL-1696)
chicken embryo, yolk sac; mouse; turkey; McCoy cells (ATCC CRL-1696)
Growth Conditions
Incubation: 5 days (approximately)
Effect on Host
Cytopathic effects (inclusions) in tissue culture
Death of embryo
Name of Depositor J Schachter
Source
turkey spleen, Texas
References

Meyer KF, Eddie B. Characteristics of a psittacosis viral agent isolated from a turkey. Proc. Soc. Exp. Biol. Med. 83: 99-101, 1953. PubMed: 13064188