Orientia chuto (ATCC® VR-1744)

Classification: Rickettsiaceae, Orientia  /  Product Format: frozen

Classification Rickettsiaceae, Orientia
Agent Orientia chuto
Strain Dubai
Biosafety Level 3
Product Format frozen
Storage Conditions  -70°C or colder
Comments
New Taxon.
It is recommended to determine viability by a PCR-based assay as agent does not produce cytopathic effects.
Effect on Host
No CPE produced
Recommended Host
Vero cells (ATCC CCL-81)

Production Host:  Vero cells (ATCC® CCL-81™)

Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and inoculate with an appropriate volume of Rickettsiae (e.g. 1 mL per 25 cm2) diluted in agent growth medium to provide an appropriate MOI (e.g. MOI of 50 for 50 Rickettsiae/ cell). Adsorb 1 hour at 800 x g at 20?C in a low speed centrifuge in closed buckets. End adsorption by adding virus growth medium to culture(s).
Incubation: Incubate infected culture(s) for 7-14 days at 35°C in a humidified 5% CO2 atmosphere.
Effect on Host
No CPE produced
Name of Depositor L Izzard
Source
Clinical female patient with acute scrub typhus infection, Dubai, United Arad Emirates, 2006.
References

Izzard L, et. al. Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai. J. Clin. Micro. 48(12): 4404-4409, 2010.