Gallid herpesvirus 2 (ATCC® VR-987)

Classification: Herpesviridae, Mardivirus  /  Product Format: frozen

Classification Herpesviridae, Mardivirus
Deposited As Marek's disease virus type 1
Agent Gallid herpesvirus 2
Strain Md-5
Biosafety Level 2
Isolation
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions
Product Format frozen
Storage Conditions  -70°C or colder
Comments
Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens.
Frozen infected cells should be thawed quickly in 37C water bath and immediately diluted in 3-5 volumes of fresh medium. After the cells attach they should be fed with fresh medium.
Highly cell associated. Serotype 1 Marek's disease virus. Considered a very virulent strain (vvMDV). Has high virulence in HVT immunized or genetically resistant chickens.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
 CPE
Recommended Host
duck embryo fibroblasts

Production Host:  DEF

chicken; chicken kidney cells; duck embryo fibroblasts
Growth Conditions
Temperature: 38.0°C
Duration: 7 days
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
 CPE
Name of Depositor RL Witter
Source
Buffy coat cells from chicken (Md.) with lymphomas, nerve lesions
References

Witter RL. . Avian Dis. 24: 210-232, 1980.