Adeno-associated virus 3 (ATCC® VR-681)

Classification: Parvoviridae, Dependovirus  /  Product Format: frozen

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Classification Parvoviridae, Dependovirus
Agent Adeno-associated virus 3
Strain H
Common Name AAV-3H
Applications
Cross-reacts with AAV-2; no hemagglutinin detected.
Biosafety Level 2
Product Format frozen
Storage Conditions  -70°C or colder
Comments
Requires co-infection with helper adenovirus Adenovirus type 2 (ATCC VR-846) for replication. Helper virus may be inactivated after harvest in water bath at 56°C for 30 minutes. Cross-reacts with AAV-2; no hemagglutinin detected.
Effect on Host
AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
Recommended Host
Production Host: HEK-293 (ATCC CRL-1573).

Production Host:  HEK-293 (ATCC® CRL-1573)

Alternate Hosts: HEK cells, KB cells (ATCC CCL-17) and HeLa cells (ATCC CCL-2)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with helper adenovirus and a small volume (e.g. 1 mL per 25 cm2) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2-3 days at 37°C temperature in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Effect on Host
AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
Name of Depositor MD Hoggan
Chain of Custody
Deposited as Adeno-associated virus type 3
Source
Adenovirus 7, SV-40 hybrid E 46 plus strain infected HEK cells
References

Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation