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Gallid herpesvirus 2

VR-1576

Product category
Viruses
Product type
Animal virus
Classification
Herpesviridae, Mardivirus
Strain designation
648A
Isolation source
Chicken blood lymphocytes.
Product format
Frozen
Storage conditions
Vapor phase of liquid nitrogen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Collect trypsinized cells from suspension by centrifugation and resuspend in chilled fresh medium supplemented with 10% serum and 10% DMSO. Controlled rate freezing is recommended.
Infect at 80-90% confluence by adding cryopreserved infected cell seed directly to culture medium.
With strains JM (v pathotype; ATCC VR-585) and Md-5 (vv pathotype; ATCC VR-987) can be used to classify new viral strains for pathotype.
Highly virulent in chickens; may produce disease in chickens vaccinated with HVT or HVT+ serotype 2 vaccines.

Characteristics

Comments
Set up cells 24 to 48 hours in advance. Infect at 80-90% confluence by adding cryopreserved infected cell seed directly to culture medium. Adsorption is not required. Incubate 4-7 days at 38° C in a humidified 5% CO2 atmosphere. Harvest by trypsinizaion of infected cell culture. Collect trypsinized cells from suspension by centrifugation and resuspend in chilled fresh medium supplemented with 10% serum and 10% DMSO. Controlled rate freezing is recommended.
Widely recognized representative of the MDV vv+ pathotype. With strains JM (v pathotype; ATCC VR-585) and Md-5 (vv pathotype; ATCC VR-987) can be used to classify new viral strains for pathotype. Highly virulent in chickens; may produce disease in chickens vaccinated with HVT or HVT+ serotype 2 vaccines. Also infects turkeys and quail. The virus is highly cell associated and must be propagated and stored as viable infected cells. A stock of infected cells at high density (107/ml) typically has an infectivity titer of about 106 PFU/ml.
Mycoplasma contamination
Not detected

Handling information

Host
Effects
cell rounding; death of host animal; foci formation; lymphoproliferative lesions in host animals; paralysis; syncytia
Complete medium
Liebovitz McCoy (ATCC® 30-2007™) medium or EMEM (ATCC® 30-2003™) with 1-4% FBS (ATCC® 30-2020™)
Temperature
38°C
Atmosphere
95% Air, 5% CO2
Incubation
4-7 days required for infection. Virus is highly cell associated and must be preserved in the form of viable cryopreserved cells.
Recommendation for infection
Set up cells 24 to 48 hours in advance.  Infect at 80-90% confluence by adding cryopreserved infected cell seed directly to culture medium.  Adsorption is not required.  Incubate 4-7 days at 38° C in a humidified 5% CO2 atmosphere.  Harvest by trypsinizaion of infected cell culture. Collect trypsinized cells from suspension by centrifugation and resuspend in chilled fresh medium supplemented with 10% serum and 10% DMSO.  Controlled rate freezing is recommended.
Handling notes
  Widely recognized representative of the MDV vv+ pathotype.  With strains JM (v pathotype; ATCC® VR-585™) and Md-5 (vv pathotype; ATCC® VR-987™) can be used to classify new viral strains for pathotype. Highly virulent in chickens; may produce disease in chickens vaccinated with HVT or HVT+ serotype 2 vaccines.  Also infects turkeys and quail.  The virus is highly cell associated and must be propagated and stored as viable infected cells.  A stock of infected cells at high density (107/mL) typically has an infectivity titer of about 106 PFU/mL.

Key abbreviations
CPE, Cytopathic effect
DMSO, Dimethyl sulfoxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum

History

Depositors
RL Witter
Type of isolate
Animal

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Witter RL. Increased virulence of Marek's disease field isolates. Avian Dis. 41: 149-163, 1997. PubMed: 9087332

Dudnikova E, et al. Evaluation of Marek's disease field isolates by the "best fit" pathotyping assay. Avian Pathology 36(2):135-143, 2007.

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