Adipocyte Differentiation Toolkit for Adipose-Derived MSCs and Preadipocytes (ATCC® PCS-500-050)

Product Format frozen 1 kit
Storage Conditions -20°C (or -70°C for long-term storage)
Applications
The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes contains medium and reagents designed to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells and Preadipocytes with high efficiency, and to support maturation of derived adipocytes during lipid accumulation. The Toolkit is composed of the following components:
  1. One vial containing 1 mL of AD Supplement for use in initiation of adipocyte differentiation
  2. One bottle containing 5 mL of ADM Supplement for use in maintaining adipocyte differentiation
  3. One bottle containing 100 mL of Adipocyte Basal Medium for use in both initiation and maintenance of adipocyte differentiation

*The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes provides enough medium and reagents for differentiation of ~6.8 x 105 cells when plated at a recommended density of 18,000 viable cells/cm2 in 4 wells of a 6 well tissue culture format. 



Biosafety Level 1
Introduction
The Adipocyte Differentiation Toolkit for Adipose-derived MSCs and Preadipocytes (ATCC PCS-500-050) contains medium and reagents designed both to induce adipogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells (ATCC PCS-500-011) and Preadipocytes (ATCC PCS-210-010) with high efficiency and to support maturation of derived adipocytes during lipid accumulation.
Quality Control Specifications Negative for bacteria, fungi and yeast.

Functional Testing Differentiation of cells into adipocytes as demonstrated by Oil Red O staining.
A Certificate of Analysis (COA) is available upon request for each lot