Perkinsus honshuensis (ATCC® PRA-177)

Organism: Perkinsus honshuensis  /  Depositor: C Dungan

Strain Designations Mie-3G/H8
Biosafety Level 2
Isolation
marine invertebrate
Mie, Japan
Isolation date: May 29, 2002
Product Format frozen
Type Strain yes
Comments
This strain is the holotype representative of Perkinsus honshuensis. This species was isolated from gill tissue from the Manila Clam, Venerupis ( = Tapes, Ruditapes) philippinarum.
Medium Medium 1886: Perkinsus broth medium
Growth Conditions
Temperature: 25.0°C
Max Temperature: 28.0°C
Min Temperature: 25.0°C
Duration: axenic
Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.
Subcultivation
Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks.
Name of Depositor C Dungan
Special Collection NSF - Protistology
Geographical Isolation Gokasho Bay
Year of Origin May 29, 2002
References

Synonym of Perkinsus olseni Lester & Davis, 1981 and P. atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus.. Bull. Eur. Ass. Fish Pathol. 22: 258-265, 2002.

Dungan C F, Reece K S. In vitro propagation of two Perkinsus spp. parasites from japanese manila clams Venerupis philippinarum, and description of Perkinsus honshuensis n. sp.. J. Eukaryot. Microbiol. in press: 2006..

Cross References

Nucleotide (GenBank) : DQ516680 LSU rRNA

Nucleotide (GenBank) : DQ516681 LSU rDNA

Nucleotide (GenBank) : DQ516682 LSU rDNA

Nucleotide (GenBank) : DQ516684 LSU rDNA

Nucleotide (GenBank) : DQ516699 ITS rDNA

Nucleotide (GenBank) : DQ516700 ITS rDNA

Nucleotide (GenBank) : DQ516701 ITS rDNA

Nucleotide (GenBank) : DQ516702 ITS rDNA

Nucleotide (GenBank) : DQ516686 Actin gene

Nucleotide (GenBank) : DQ516687 Actin gene

Nucleotide (GenBank) : DQ516688 Actin gene

Nucleotide (GenBank) : DQ516689 Actin gene

Nucleotide (GenBank) : DQ516690 Actin gene

Nucleotide (GenBank) : DQ516691 Actin gene

Nucleotide (GenBank) : DQ516692 Actin gene