Neospongiococcum excentricum Deason and Bold (ATCC® 74108)

Organism: Neospongiococcum excentricum Deason and Bold  /  Depositor: Coors BioTech, Inc., SA Orndorff, Coors BioTech, Inc.

Strain Designations HZ-1236/274
Application
produces zeaxanthin and other carotenoids
Biosafety Level 1
Isolation
mutant derived from ATCC 40335
Type Strain no
Medium ATCC® Medium 2085: YEP medium
Growth Conditions
Temperature: 30.0°C
Duration: axenic
Protocol: This strain is routinely shipped as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the entire contents to 5 ml of ATCC medium 200 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen it by one half turn, and incubate the tube on a horizontal slant (5-15 degree slant) at 30C. The culture should be ready to subculture in 5-7 days. To subculture, crew the cap on tightly, agitate the tube vigorously, and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2085. Incubate as above.
Subcultivation
Protocol: This strain is routinely shipped as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the entire contents to 5 ml of ATCC medium 200 in a 16 x 125 mm screw-capped test tube. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw the cap on tightly, loosen it by one half turn, and incubate the tube on a horizontal slant (5-15 degree slant) at 30C. The culture should be ready to subculture in 5-7 days. To subculture, crew the cap on tightly, agitate the tube vigorously, and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 2085. Incubate as above.
Name of Depositor Coors BioTech, Inc., SA Orndorff, Coors BioTech, Inc.
References

Orndorff SA, et al. Zeaxanthin producing strains of Neospongiococcum excentricum. US Patent 5,360,730 dated Nov 1 1994