pSYX34lacZalpha (ATCC® 87220)

Applications: vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: S Xu

Permits and Restrictions

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Designations pSYX34lacZalpha
Depositors S Xu
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.5000000000000000
Vector: pSYX34lacZalpha (plasmid)
Construction: pSC101, pACYC184, pUC19
Marker(s):cmlR
Construct size (kb): 6.5
Features: marker(s): cmlR
replicon: pSC101
MCS: HindIII...SacI
coding sequence: lacZalpha
Applications
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; HindIII--5.4; PstI--5.4.
A low copy-number vector compatible with plasmids having pMB1 or p15A origins of replication. Permits blue/white screening for recombinants.
The copy number is estimated at 10 copies per cell.
Recommendation for verification: BamHI; HindIII; PstI.
Media ATCC Medium 1065 (see below) plus 30.0 mcg/ml chloramphenicol ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References

Xu SY, Fomenkov A. Construction of pSC101 derivatives with Camr and Tetr for selection or LacZ for blue/white screening. BioTechniques 17: 57, 1994. PubMed: 7946313

Shuang-yong Xu, personal communication

Shipped freeze-dried