pSYX34lacZalpha (ATCC® 87220)

Applications: vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: S Xu

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Designations pSYX34lacZalpha
Depositors S Xu
Biosafety Level 1
Vector Information
Size (kb): 6.5000000000000000
Vector: pSYX34lacZalpha (plasmid)
Construction: pSC101, pACYC184, pUC19
Marker(s):cmlR
Construct size (kb): 6.5
Features: marker(s): cmlR
replicon: pSC101
MCS: HindIII...SacI
coding sequence: lacZalpha
Applications
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; HindIII--5.4; PstI--5.4.
A low copy-number vector compatible with plasmids having pMB1 or p15A origins of replication. Permits blue/white screening for recombinants.
The copy number is estimated at 10 copies per cell.
Recommendation for verification: BamHI; HindIII; PstI.
Media ATCC Medium 1065 (see below) plus 30.0 mcg/ml chloramphenicol ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References

Xu SY, Fomenkov A. Construction of pSC101 derivatives with Camr and Tetr for selection or LacZ for blue/white screening. BioTechniques 17: 57, 1994. PubMed: 7946313

Shuang-yong Xu, personal communication

Shipped freeze-dried
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