pOSEX3 (ATCC® 87212)

Applications: expression vector  /  Depositors: E Bremer

Permits and Restrictions

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Designations pOSEX3
Depositors E Bremer
Biosafety Level 1
Vector Information
Size (kb): 4.4320001602172850
Vector: pOSEX3 (plasmid)
Promoters: Promoter for expression proU
Construction: pOSEX2
Marker(s):ampR
Construct size (kb): 4.432000160217285
Features: marker(s): ampR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: BamHI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Vector does not provide a ribosome binding site or initiation codon.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Vector constructed from pOSEX2 (ATCC 87211) by deletion of a portion of the pBR322 backbone.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862

Erhard Bremer, personal communication

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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