pBSL159 (ATCC® 87148)

Applications: contains easily purifiable cassette(s) for construction omega - Ap  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL159
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.1440000534057620
Vector: pBSL159 (plasmid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pSU18
Construct size (kb): 4.144000053405762
Features: marker(s): cmlR
promoter for in vitro transcription: T7 (phi10)
replicon: p15A
MCS: KpnI...SacI MluI
MCS: MluI SacI...KpnI
coding sequence: bla (ampR)
contains easily purifiable cassette(s) for construction omega - Ap
Restriction digests of the clone give the following sizes (kb): BamHI--2.1, 2.0; EcoRI--2.1 (doublet); KpnI--2.15, 2.0; MluI--2.2, 2.0; SstI--2.15, 2.0.
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Ap element flanked by multiple cloning sites duplicated in inverted orientation.
The PstI and HincII sites in the bla gene have been removed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried