pBSL128 (ATCC® 87144)

Applications: contains easily purifiable cassette(s) for construction omega-nptII  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL128
Depositors MF Alexeyev
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.0599999427795410
Vector: pBSL128 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,kanR
Construct size (kb): 5.059999942779541
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: nptII (kanR)
Applications
contains easily purifiable cassette(s) for construction omega-nptII
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.0; EcoRI--2.9, 2.1; KpnI--2.9, 2.0; MluI--3.0, 2.0; SstI--3.0, 2.0.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
Plasmid useful for vector construction. Contains a nptII (kanR) cassette flanked by omega elements and multiple cloning sites duplicated in inverted orientation.
SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
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