pPB1 (ATCC® 77473)

Applications: expression vector  /  Depositors: DR Tolan

Permits and Restrictions

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Designations pPB1
Depositors DR Tolan
Biosafety Level 1
Vector Information
Size (kb): 2.6699988842010500
Vector: pPB1 (plasmid)
Promoters: Promoter trc
Construction: pUC19, pKK233-2
Marker(s):ampR
Construct size (kb): 2.66999888420105
Features: marker(s): ampR
promoter: trc
replicon: pMB1
transcription terminator: rrnB
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; HindIII--2.7; NcoI--2.7.
Expression vector. Permits high copy number and production of proteins initiated at the ATG of beta-galactosidase.
The order of the major features in the plasmid is: pMB1 ori - EcoRI - trc promoter - lacZ'/NcoI - PstI - HindIII - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Beernink PT, Tolan DR. Construction of a high-copy "ATG vector" for expression in Escherichia coli. Protein Expr. Purif. 3: 332-336, 1992. PubMed: 1422227

Shipped frozen
Shipping Information Frozen glycerol stock of  E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet