pPB1 (ATCC® 77473)

Applications: expression vector  /  Depositors: DR Tolan

Permits and Restrictions

View Permits

Designations pPB1
Depositors DR Tolan
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.6699988842010500
Vector: pPB1 (plasmid)
Promoters: Promoter trc
Construction: pUC19, pKK233-2
Marker(s):ampR
Construct size (kb): 2.66999888420105
Features: marker(s): ampR
promoter: trc
replicon: pMB1
transcription terminator: rrnB
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; HindIII--2.7; NcoI--2.7.
Expression vector. Permits high copy number and production of proteins initiated at the ATG of beta-galactosidase.
The order of the major features in the plasmid is: pMB1 ori - EcoRI - trc promoter - lacZ'/NcoI - PstI - HindIII - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Beernink PT, Tolan DR. Construction of a high-copy "ATG vector" for expression in Escherichia coli. Protein Expr. Purif. 3: 332-336, 1992. PubMed: 1422227

Shipped frozen
Shipping Information Frozen glycerol stock of  E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet