pHCMV-G (ATCC® 75497)

Applications: cloning vector  /  Depositors: Regents of the University of California, J Yee, JC Burns, Regents of the University of California

Permits and Restrictions

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Designations pHCMV-G
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors Regents of the University of California, J Yee, JC Burns, Regents of the University of California
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.3499999046325680
Intact vector size: 6.350
Type of vector: plasmid
Cloning sites: XbaI, SalI, BamHI, XhoI, KpnI
Polylinker sites:
Construction: pBR322, HCMV, VSV-G
Host range: Homo sapiens; hamster; zebrafish; Escherichia coli
Features (with orientation and position when available):
promoter: HCMV (cytomegalovirus), ->
marker(s): ampR, ->
Vector: pHCMV-G (plasmid)
Promoters: Promoter HCMV (cytomegalovirus)
Construction: pBR322, HCMV, VSV-G
Construct size (kb): 6.349999904632568
Features: marker(s): ampR
promoter: HCMV (cytomegalovirus)
cloning vector
The retrovirus-derived vectors in which the retroviral envelope glycoprotein has been replaced by the G glycoprotein of vesicular stomatitis virus. Vector can be used in the transfer of an exogenous gene into the cells of non-mammalian organisms.
Contains the immediate early promoter of human cytomegalovirus, the cDNA encoding VSC-G protein and the polyademylation signal of the rabbit B-globin gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Burns JC. Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors. US Patent 5,512,421 dated Apr 30 1996

Shipped frozen
Shipping Information Distributed: DNA supplied by depositor (500 ng dried)
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