pSM147 (ATCC® 53040)

Applications: expression vectorshuttle vector  /  Depositors: G Grandi

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Designations pSM147
Depositors G Grandi
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 6.5999999046325680
Vector: pSM147 (plasmid)
Promoters: Promoter synthetic
Construction: pUB110, pBR322 ori, fd terminator
Marker(s):ampR,kanR
Construct size (kb): 6.599999904632568
Features: marker(s): ampR, kanR
promoter: synthetic
replicon: pMB1, pUB110
terminator: fd
Applications
expression vector
shuttle vector
Comments
An Escherichia coli/Bacillus subtilis shuttle expression vector utilizing a synthetic promoter and an fd terminator.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. Japanese Patent 61,257,189 dated Nov 14 1986

Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. European Patent 195303 dated Sep 24 1986

Grandi G, et al. Plasmid vector containing erythromycin promoter of pE 194 modified by replacement of minus 35 region to increase expression of heterologous protein. German Federal Republic Patent 3,666,838 dated Dec 14 1989

Guido Grandi, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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Product Sheet
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