pAbT4674 (ATCC® 40829)

Applications: The vector contains the gag-pol gene under the control of the vaccinia 40K promoter and the env gene under the control of the vaccinia D1 promoter.  /  Depositors: Therion Biologics Corporation, GP Mazzara, Therion Biologics Corporation

Permits and Restrictions

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Designations pAbT4674
Depositors Therion Biologics Corporation, GP Mazzara, Therion Biologics Corporation
Biosafety Level 1
Vector Information
Size (kb): 12.0640001296997100
Vector: pAbT4674 (plasmid)
Promoters: Promoter vaccinia D1
Marker(s):ampR
Construct size (kb): 12.06400012969971
Features: marker(s): ampR
promoter: vaccinia 40K promoter
promoter: vaccinia D1
replicon: pMB1
coding sequence: HIV-1 env gene
coding sequence: HIV-1 gag-pol gene
coding sequence: vaccinia 29K host-range gene
Applications
The vector contains the gag-pol gene under the control of the vaccinia 40K promoter and the env gene under the control of the vaccinia D1 promoter.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.9, 3.0, 1.1; PstI--9.2, 2.0, 0.8
The vector contains the gag-pol gene under the control of the vaccinia 40K promoter and the env gene under the control of the vaccinia D1 promoter.
The vector is for the insertion and expression of HIV gag-pol (strain HXB2) and env (strain BH10) in vaccinia virus.
References

Mazzara GP, et al. Self-assembled, defective, non-self-propagating lentivirus particles. US Patent 5,614,404 dated Mar 25 1997

Shipped freeze-dried
Shipping Information Distributed: DNA supplied by depositor (dried) (amount: 2 ug)
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 40829