pPLOP (ATCC® 39947)

Applications: expression vector  /  Depositors: Cetus Corp., A Belt, Cetus Corp.

Permits and Restrictions

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Designations pPLOP
Depositors Cetus Corp., A Belt, Cetus Corp.
Biosafety Level 1
Host
Distribution host: Escherichia coli CMCC 2118
Vector Information
Size (kb): 0E-16
Vector: pPLOP (plasmid)
Promoters: Promoter lambda PL
Construction: pFC5(EcoRI/HindIII), pCS3
Marker(s):ampR
Construct size (kb): 0.0
Features: marker(s): ampR
promoter: lambda PL
replicon: pEW27
Applications
expression vector
Comments
A lambda PL expression plasmid vector with temperature sensitive replication system (high copy number at high temperature).
LambdaPL promoter is upstream of HindIII site and downstream of BamHI site. The HindIII site is 3' of the N gene ribosome binding site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,063 dated Jun 30 1987

Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,064 dated Jun 30 1987

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet