pEX11 (ATCC® 37708)

Applications: expression vectorvector for constructing cDNA libraries  /  Depositors: BA Van Der Zeijst

Permits and Restrictions

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Designations pEX11
Depositors BA Van Der Zeijst
Biosafety Level 1
Vector Information
Size (kb): 5.8000001907348630
Vector: pEX11 (plasmid)
Promoters: Promoter lambda PR
Construction: pEX1, oligonucleotide
Marker(s):ampR
Construct size (kb): 5.800000190734863
Features: marker(s): ampR
promoter: lambda PR
replicon: pMB1
terminator: phage fd
enhancer: none
Applications
expression vector
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8; BamHI--5.8; PstI--5.8; EcoRI/EcoRV--4.3, 1.9; SalI--5.8.
This strain is resistant to 100 ug/ml ampicillin.
The recombinant fusion protein generated using this vector is not solubilized in Triton X-100 lysis procedures and is thus easily purified, resistant to proteolysis, and easily detected by direct immunoscreening.
This is one of three bacterial expression vectors (pEX11, ATCC 37708; pEX12, ATCC 37709; and pEX13, ATCC 37710) which differ by translational reading frames and which express cro-lacZ fusion proteins regulated by the PR promoter of bacteriophage lambda.
This was constructed from pEX1 by the ligating a synthetic oligonucleotide into pEX1 cut with XmaI and BamHI. The oligonucleotide added several unique restriction sites.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Kusters JG, et al. Improvement of the cloning linker of the bacterial expression vector pEX. Nucleic Acids Res. 17: 8007, 1989. PubMed: 2678010

Stanley KK, Luzio JP. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO J. 3: 1429-1434, 1984. PubMed: 6086324

Bernard A Van Der Zeijst, personal communication

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