pSP64-f1- (ATCC® 37361)

Applications: vector permitting RNA synthesis in vitrovector permitting production of single-stranded DNA  /  Depositors: DA Mead

Permits and Restrictions

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Designations pSP64-f1-
Depositors DA Mead
Biosafety Level 1
Vector Information
Size (kb): 3.5000000000000000
Vector: pSP64-f1- (phagemid)
Promoters: Promoter SP6
Construction: bacteriophage f1, pSP64
Marker(s):ampR
Construct size (kb): 3.5
Features: marker(s): ampR
promoter: SP6
replicon: pMB1, f1
Applications
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
Comments
M9 medium is required to retain f1.
This plasmid vector can be used for in vitro RNA synthesis from the SP6 promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
M9
References

Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795

Shipped freeze-dried
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