pPL703 (ATCC® 37219)

Applications: promoter-cloning vectorshuttle vector  /  Depositors: PS Lovett

Permits and Restrictions

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Designations pPL703
Depositors PS Lovett
Biosafety Level 1
Vector Information
Size (kb): 4.5000000000000000
Vector: pPL703 (plasmid)
Promoters: Promoter none
Construction: pPL603, M13mp7 polylinker
Marker(s):neoR
Construct size (kb): 4.5
Features: insert detection: CAT
marker(s): neoR
promoter: none
replicon: pUB110
Applications
promoter-cloning vector
shuttle vector
Comments
The PstI site is 144 bp upstream from cat-86.
Cat-86 is not expressed until a promoter-containing fragment is cloned into one of the polylinker sites.
A Bacillus subtilis promoter cloning plasmid vector.
Media ATCC® Medium 1179: VY medium
Growth Conditions
Temperature: 37.0°C
References

Duvall EJ, et al. Chloramphenicol-inducible gene expression in Bacillus subtilis. Gene 24: 171-177, 1983. PubMed: 6416927

Mongkolsuk S, et al. Restriction fragments that exert promoter activity during postexponential growth of Bacillus subtilis. J. Bacteriol. 155: 1399-1406, 1983. PubMed: 6309749

Shipped freeze-dried
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