YIp33 [pRB19] (ATCC® 37064)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YIp33 [pRB19]
Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 6.6999998092651370
Vector: YIp33 (plasmid)
Construction: pBR322, pYe(Leu2)10
Marker(s):LEU2,ampR
Construct size (kb): 6.699999809265137
Features: marker(s): ampR, LEU2
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
This was constructed by inserting a 2.2 kb SalI/XhoI fragment containing LEU2 from pYe(Leu2)10 into the SalI site of pBR322. The 5' - 3' orientation of LEU2 is counterclockwise. LEU2, originating from strain X2180-1A, includes a region of Ty1-17.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

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