YIp33 [pRB19] (ATCC® 37064)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YIp33 [pRB19]
Depositors D Botstein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.6999998092651370
Vector: YIp33 (plasmid)
Construction: pBR322, pYe(Leu2)10
Marker(s):LEU2,ampR
Construct size (kb): 6.699999809265137
Features: marker(s): ampR, LEU2
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
This was constructed by inserting a 2.2 kb SalI/XhoI fragment containing LEU2 from pYe(Leu2)10 into the SalI site of pBR322. The 5' - 3' orientation of LEU2 is counterclockwise. LEU2, originating from strain X2180-1A, includes a region of Ty1-17.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

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