2CEN (ATCC® 87411)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

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Designations 2CEN
Species Homo sapiens, human
Depositors F Kao
Origin
lymphoblast cell line GM03714 (46, XX)
Applications
Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes.
Vector
Tissue: lymphoblast cell line GM03714 (46, XX)
Chromosome: 2p11-q11
DNA: genomic
Digest: Complete
Insert size range: 50-600 bp
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes.
The library covers the region 2p11.1-2q11.1.
Approximately 50% of the clones contain repetitive sequences. Of 46 single copy clones tested, 37 (80%) hybridized to chromosome 2.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.
References

Kao FT, et al. Complete set of eleven region-specific microdissection libraries for human chromosome 2. Somatic Cell Mol. Genet. 22: 57-66, 1996. PubMed: 8643994

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