lambdaACT - mouse pre-B cell cDNA (ATCC® 87292)

Organism: Mus musculus, mouse  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaACT - mouse pre-B cell cDNA
Species Mus musculus, mouse
Depositors SJ Elledge
Origin
lymphocyte, pre-B
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaACT
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Construction: lambdaYES, pSE1107
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
restriction site: NotI, ->
other: lox, ->
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
promoter: ADH, ->
other: GAL4 activation domain, ->
MCS: BglII/EcoRI...XhoI/BglII, ->
terminator: ADH, ->
other: lox, ->
restriction site: NotI
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
Tissue: lymphocyte, pre-B
DNA: cDNA
Insert size range: 0.7 - 7 kb
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

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