lambdaACT - Schizosaccharomyces pombe cDNA (ATCC® 87289)

Organism: Schizosaccharomyces pombe Lindner, teleomorph, fission yeast  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

View Permits

Designations lambdaACT - Schizosaccharomyces pombe cDNA
Species Schizosaccharomyces pombe Lindner, teleomorph, fission yeast
Depositors SJ Elledge
Vector
Marker(s):LEU2,ampR
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaACT
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Construction: lambdaYES, pSE1107
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
restriction site: NotI, ->
other: lox, ->
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
promoter: ADH, ->
other: GAL4 activation domain, ->
MCS: BglII/EcoRI...XhoI/BglII, ->
terminator: ADH, ->
other: lox, ->
restriction site: NotI
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
DNA: cDNA
Cloning site: XhoI
Insert size range: 0.7 - 7 kb
Insert ends: XhoI adaptors
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

recommended for amplification

recommended for cre-lox based conversion to plasmid

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