lambdaACT - Schizosaccharomyces pombe cDNA (ATCC® 87289)

Organism: Schizosaccharomyces pombe Lindner, fission yeast  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaACT - Schizosaccharomyces pombe cDNA
Species Schizosaccharomyces pombe Lindner, fission yeast
Depositors SJ Elledge
Name of vector: lambdaACT
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Construction: lambdaYES, pSE1107
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
restriction site: NotI, ->
other: lox, ->
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
promoter: ADH, ->
other: GAL4 activation domain, ->
MCS: BglII/EcoRI...XhoI/BglII, ->
terminator: ADH, ->
other: lox, ->
restriction site: NotI
other: right arm (15.2 kb, with cI857)
Cross references:
Cloning site: XhoI
Insert size range: 0.7 - 7 kb
Insert ends: XhoI adaptors
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

recommended for amplification

recommended for cre-lox based conversion to plasmid

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