lambdaYES - mouse T cell cDNA (ATCC® 87272)

Organism: Mus musculus, mouse  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaYES - mouse T cell cDNA
Species Mus musculus, mouse
Depositors SJ Elledge
Origin
T lymphocyte, VL3 cell line
Applications
To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, use of BNN132 (ATCC 47059) is recommended.
Vector
Tissue: T lymphocyte, VL3 cell line
DNA: cDNA
Insert size range: 0.7 - 7 kb
Insert ends: XhoI adaptors
Number of independent recombinants: 1 X 10^8
Nonrecombinants (%): 5.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

 Frozen stock of bacteria-free phage lysate.

                   

Comments
To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, use of BNN132 (ATCC 47059) is recommended.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

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