Xenopus laevis NotI/SalI (ATCC® 86985)

Organism: Xenopus laevis, frog, South African clawed  /  Clone Type: Library  /  Depositors: MJ Castanon

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Designations Xenopus laevis NotI/SalI
Species Xenopus laevis, frog, South African clawed
Depositors MJ Castanon
Origin
oocyte
Vector
Tissue: oocyte
DNA: cDNA
Insert size range: 1 - 2 kb
Genome Equivalent: 0.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information

 Frozen glycerol stock of library in Escherichia coli HB101.

Comments
Constructed using a SalI-containing oligo(dT) primer and adaptors to result in 5'NotI and 3'SalI ends.
pMC944 is a YE-type vector permitting expression from the mating factor alpha 1 promoter. Unique XbaI and SphI sites in the promoter and ADH2 terminator, respectively, allow transfer of the expression cassette.
The vector does not contain an initiation codon. It must be provided by the cDNA.
Verified by PCR to contain clones for B4, D7, enolase, Eg1, and nucleoplasmin. The low abundance Vg1 was not detected.
References

Spevak W, et al. Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats. Mol. Cell. Biol. 13: 4953-4966, 1993. PubMed: 8393141

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