LI1 (ATCC® 77426)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

Permits and Restrictions

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Designations LI1
Species Homo sapiens, human
Depositors A Swaroop
Origin
large intestine, 14 months
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:
Vector
Tissue: large intestine, 14 months
DNA: cDNA
Insert size range: > 0.8 kb
Genome Equivalent: 0.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
Comments
Titer determined on E. coli LE392.
cDNA synthesis was primed by oligo-dT, and EcoRI linkers were added.
References

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

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