LL22NL02 (ATCC® 57779)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: P De Jong, JA Garnes

Permits and Restrictions

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Designations LL22NL02
Species Homo sapiens, human
Depositors P De Jong, JA Garnes
Origin
hybrid J460-51 cell line
Vector
Tissue: hybrid J460-51 cell line
Chromosome: 22
DNA: genomic
Digest: Partial
Insert size range: 15 kb average
Genome Equivalent: 15.0
Coverage: Clones for "complete" coverage: 4500.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Purity (human clones): 83%. Contaminants (hamster): 3%.
The J640-51 cell line was provided by C. Jones, Eleanor Roosevelt Institute of Cancer Research, Denver, CO 80206. It is described in Cytogenet. Cell Genet. 28: 181-194, 1980 and Somat. Cell. Mol. Genet. 17: 117-136, 1991.
Chromosomes for library construction were isolated by Ger van der Engh using flow-sorting.
Genomic equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 54 Mb/chromosome 22.
References

Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608

Pieter De Jong, personal communication

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