LL18NS01 (ATCC® 57710)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

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Designations LL18NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
skin fibroblast 761 cell line
Tissue: skin fibroblast 761 cell line
Chromosome: 18
DNA: genomic
Digest: Complete
Insert size range: 2.0 kb average
Genome Equivalent: 72.0
Coverage: Clones for "complete" coverage: 12000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Good resolution and well-isolated #18 peak. Purity estimated by banding: 97%.
Of 20 probes selected at random, 6 inserts were smaller than 300 bp, 11 contained repetitive sequences, and 3 contained unique sequences. Of the latter 3, all mapped to chromosome 18, and 1 contained 3 HindIII fragments contiguous in the genome.

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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