(ATCC® 37483)

Organism: Bos taurus, cow  /  Clone Type: Library  /  Depositors: R Reeves

Permits and Restrictions

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Species Bos taurus, cow
Depositors R Reeves
Origin
lymphocyte, Con A/PMA-stimulated, 15 hr
Applications
EcoRI linkers were used in the construction.
Vector
Tissue: lymphocyte, Con A/PMA-stimulated, 15 hr
DNA: cDNA
Insert size range: > 0.2 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 200000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Amplified on E. coli Y1088. Titer on Y1090. Wild-type (non-recombinants) lambdagt11 will amplify more than recombinants. The ratio after first amplification changed from 80% recombinant to 50% recombinant.
EcoRI linkers were used in the construction.
References

Young RA, Davis RW. Yeast RNA polymerase II genes: isolation with antibody probes. Science 222: 778-782, 1983. PubMed: 6356359

Reeves R, et al. Molecular cloning of a functional bovine interleukin 2 cDNA. Proc. Natl. Acad. Sci. USA 83: 3228-3232, 1986. PubMed: 3486415

Product Sheet
Product Sheet
Product Sheet