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pWR3.26 [molecular clone of rhinovirus 14] purified plasmid DNA

VRMC-7

Name:  pWR3.26
Size (kb):  9.847 kb
Original source: sample of HRV14 strain 1059 donated by V.V. Hamparian
Organism
Human rhinovirus 14
Classification
Picornaviridae, Enterovirus, Rhinovirus B
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence

Characteristics

Comments
The construct contains a full-length Human Rhinovirus type 14 (HRV14) genome cloned into the StuI site of the pMJ3 vector.
HRV14 cDNA was transplanted into the vector at the StuI site.
The 5' end of the cDNA is two bases (GG) from the transcription initiation site of the T7 promoter. A unique MluI site (ACGCGT) was added after the polyA tail. Full length transcripts can be produced using T7 RNA polymerase. T7 transcripts are half as infective as genomic RNA from virons.
Mycoplasma contamination
Not detected

Vector information

Vector name
pUC19
Type of vector
E. coli
Cloning sites
The HRV14 cDNA was transplanted into the vector at the StuI site, then additional modifications were made to the viral sequence by site-directed mutagenesis.
Markers
ampR

Insert information

Insert information
Markers (genes and/or restriction sites): HindIII - 3 sites; Fragment lengths: 5338, 2362, 2147
MluI- 1 site; Fragment length: 9847
Insert description (full-length, etc.): full-length HRV 14cDNA and T7 promoter
Full/partial sequence available (ie. GenBank #): L05355 (viral sequence)

Handling information

Host
Escherichia coli
Handling procedure
Rehydrate plasmid with water or TE buffer. Plasmid can be transformed into a suitable Escherichia coli host using standard protocols and then grown on LB + 50 mg/mL of ampicillin agar. Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures.

Handling notes
This construct contains a 42 bp poly(A) tail.

History

Deposited as
Human rhinovirus type 14
Depositors
W Lee
Special collection
NCRR Contract
Cross references
GenBank L05355 Human rhinovirus type 14 polyprotein gene.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Lee WM, et al. Role of maturation cleavage in infectivity of picornaviruses: activation of an infectosome. J. Virol. 67: 2110-2122, 1993. PubMed: 8383233

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