LentiLox 3.7 [pLL3.7] (ATCC® VRMC-39)

Applications: This material is distributed by ATCC under the terms of the ATCC Material Transfer Agreement (MTA) substantially amended by the MIT Cre-Lox MTA that allows use only for non-commercial purposes.  /  Depositors: L Van Parijs

Designations LentiLox 3.7 [pLL3.7]
Depositors L Van Parijs
Biosafety Level 1
Host
E. coli
Vector Information
Size (kb): 7.65

        Description:  Self-inactivating lentivirus vector (SIN vector).  Highly modified, partial HIV type 1 genome containing a CMV driven EGFP reporter and a U6 promoter upstream of cloning sites for shRNA expression. 

Vector: LentiLox 3.7 (plasmid)
Type: expression
Construction: pUC plus CMV driven EGFP reporter, U6 promoter, and additional polylinker.
Marker(s): AmpR
Features: marker(s): AmpR
promoter: U6, CMV
Applications
This material is distributed by ATCC under the terms of the ATCC Material Transfer Agreement (MTA) substantially amended by the MIT Cre-Lox MTA that allows use only for non-commercial purposes.
Comments
Self-inactivating lentivirus vector (SIN vector). Highly modified, partial HIV type 1 genome containing a CMV driven EGFP reporter and a U6 promoter upstream of cloning sites for shRNA expression. The pLL3.7 map and sequence are available. This material is distributed by ATCC under the terms of the ATCC Material Transfer Agreement (MTA) substantially amended by the MIT Cre-Lox MTA that allows use only for non-commercial purposes. Additionally, the use of this material is restricted by US patent 4,959,317. For-profit, commercial institutions must obtain a license agreement prior to purchase. For instructions on how to proceed, please contact ATCC's Office of Licensing and Business Development at licensing@atcc.org.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Growth Condition: grows in E. coli strain DH5alpha-T1
References

or colder

Rubinson DA, et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat. Genet. 33: 401-406, 2003. PubMed: 12590264

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