pCW8 plasmid in E. coli (ATCC® MBA-86)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Depositors: R. Kopito

Permits and Restrictions

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Designations pCW8 plasmid in E. coli
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors R. Kopito
Vector
Construct size (kb): 4.136
Vector: pRBG4
Vector size (kb): 3.806
Insert
Ubiquitin, mutant
Insert size (kb): 0.330 (Ubiquitin, 6HIS and c-myc tag)
Insert site: ClaI/EcoRI
Excise insert: EcoRI
Putative ID
ubiquitin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
This is a cDNA clone of ubiquitin K48R from Saccharomyces cerevisiae with a 6 HIS, c-myc N-terminal tag cloned into the ClaI/EcoRI sites of the pRBG4 vector. The plasmid was transformed into E. coli DH10B.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Ward CL, et al. Degradation of CFTR by the ubiquitin-proteasome pathway. Cell 83: 121-127, 1995.